The Berthold Technologies Mithras LB 940 microplate reader delivers high-sensitivity detection for life science assays, supporting multi-mode measurements across 340–750 nm. Designed for demanding research environments, this instrument combines flexible optics with intuitive workflow controls.
Below you will find a detailed overview of key capabilities, performance highlights, integration options, and practical guidance for evaluating the Mithras LB 940 in your laboratory.
| Model | Detection Range (nm) | Read Modes | Throughput (96-well) |
|---|---|---|---|
| Mithras LB 940 | 340–750 | Absorbance, Fluorescence, Luminescence, FRET, TR-FRET | <15 min |
High-Sensitivity Assay Performance
Ultraviolet to Near-Infrared Flexibility
The Mithras LB 940 covers 340–750 nm with dual monochromators, enabling precise endpoint and kinetic measurements. Users benefit from low background noise and high signal-to-noise ratios even in challenging assays.
Microplate Format Versatility
Compatible with 6–384 well plates, deep and standard microplates, the system adapts to screening campaigns, pilot studies, and method development. Flexible injection ports accommodate reagent additions without compromising plate position accuracy.
Assay Development and Screening Applications
Cell-Based and Biochemical Assays
Researchers rely on the Mithras LB 940 for reporter gene, viability, and proliferation assays, as well as enzyme kinetics and binding studies. The instrument supports label-free and labeled detection across diverse protocols.
High-Throughput Screening Efficiency
Automated plate handling, rapid read times, and advanced data processing contribute to higher throughput per hour. Integrated software features simplify plate layout design, scheduling, and result reporting.
System Integration and Laboratory Workflow
Data Management and Connectivity
Built-in networking options support seamless data transfer to LIMS and network folders. Audit trails and electronic signature capabilities help meet regulatory expectations for method traceability and data integrity.
Installation and Operational Support
Berthold provides installation qualification guidance, method transfer assistance, and training tailored to user proficiency levels. Service agreements and remote diagnostics help minimize downtime and sustain performance.
Technical Specifications and Performance
| Parameter | Specification | Advantage |
|---|---|---|
| Wavelength Range | 340–750 nm | Broad detection for absorbance and fluorescence |
| Read Modes | Absorbance, Fluorescence, Luminescence, FRET, TR-FRET | Supports diverse assay formats |
| Dynamic Range | High | Accurate quantitation across wide concentration ranges |
| Throughput | <15 min per 96-well plate | Enables rapid screening campaigns |
FAQ
What types of assays are validated on the Mithras LB 940 microplate reader lab configuration?
The system is validated for absorbance, fluorescence, and luminescence assays, including endpoint and kinetic measurements, FRET, and TR-FRET workflows across research applications.
How does the dual monochromator design improve data quality in the Mithras LB 940 experiments?
Dual monochromators provide precise wavelength selection, high sensitivity, and low background, which enhance signal accuracy and reproducibility for demanding assays.
Can the Mithras LB 940 integrate with existing laboratory information systems in a regulated environment?
Yes, the reader supports secure data export, network connectivity, and audit trails to align with regulatory expectations for data integrity and traceability.
What support options are available for method development and qualification on the Berthold Technologies Mithras LB 940 lab instrument?
Berthold offers installation qualification, method transfer assistance, training programs, and service agreements tailored to ensure reliable performance and compliance.
Optimizing Workflow with the Mithras LB 940 Platform
- Leverage dual monochromator optics for sensitive and reproducible measurements across 340–750 nm.
- Standardize plate workflows to maximize throughput and minimize variability between runs.
- Configure data export settings early to ensure compatibility with LIMS and regulatory requirements.
- Utilize remote diagnostics and scheduled maintenance to sustain long-term performance.
- Plan method transfer and validation with Berthold support to streamline qualification and reduce downtime.