cd61 integrin beta 3 antibody functional grade 16061182 supports high-sensitivity detection of CD61 in flow cytometry, immunofluorescence, and Western blot applications. This functional grade reagent is designed for consistent performance in cell adhesion, migration, and signaling studies.
Engineered to meet stringent quality criteria, the antibody targets the beta-3 subunit of integrin CD61, enabling precise quantification of integrin alpha-IIb/beta-3 and beta-3/alpha-5 complexes in hematopoietic and endothelial cell models.
Product Specification Overview
Key parameters and validation metrics for cd61 integrin beta 3 antibody functional grade 16061182 are summarized in the table below.
| Attribute | Details | Validation | Typical Storage |
|---|---|---|---|
| Target | CD61 (Integrin Beta-3) | Recombinant protein epitope mapping | Lyophilized pellet |
| Clone | 16061182 | In-house epitope competition assay | -20°C |
| Reactivity | Cross-species ELISA and flow cytometry | 4°C up to 4 weeks post reconstitution | |
| Format | Purified IgG | Batch-specific SDS-PAGE verification | Aliquot and avoid freeze-thaw cycles |
| Applications | Flow Cytometry, Immunofluorescence, Western Blot | Published dataset alignment with internal controls | Refer to Certificate of Analysis |
Integrin Biology and Cell Adhesion Context
CD61, the beta-3 subunit, forms heterodimers with alpha-IIb in platelets and alpha-V, alpha-5, and alpha-6 in diverse tissues. These integrins regulate focal adhesion formation, mechanotransduction, and outside-in signaling critical for hemostasis and tumor progression.
The cd61 integrin beta 3 antibody functional grade 16061182 has been optimized to recognize conformational epitopes exposed in resting and activated states, supporting multiplex assays alongside phospho-specific markers of adhesion kinase and paxillin.
Functional Grade Performance and Quality Control
Titer and Sensitivity
Internal lot testing confirms high titer in human PBMCs and endothelial cell lines, with minimal background in isotype controls and reliable detection down to 10 ng protein per well in lysates.
Batch Consistency and Reproducibility
Each functional grade shipment undergoes automated microtiter plate adhesion assays, flow cytometry gating stability checks, and Western blot reproducibility trials to ensure lot-to-lot performance aligns with project-specific requirements.
Advanced Applications and Multiplex Workflow Integration
Researchers routinely deploy cd61 integrin beta 3 antibody functional grade 16061182 in multicolor flow panels that include CD41, CD45, and phospho-FAK. This enables simultaneous phenotyping and functional readouts of adhesion dynamics in circulating monocytes and endothelial progenitors.
In three-dimensional matrix models, the antibody supports collagen I and Matrigel invasion assays when combined with confocal z-stack scanning, revealing migratory paths and focal complex maturation dependent on beta-3 integrin avidity switching.
Experimental Considerations and Protocol Optimization
Optimal permeabilization conditions vary across fixation agents; empirical testing with saponin versus methanol protocols is recommended for intracellular epitope access. Paired isotype controls and fluorescence minus one sets aid confident interpretation in high-dimensional immunophenotyping.
For Western blot applications, reduced conditions and careful blocking minimize heavy chain aggregation, while pre-clearing with protein A/G resin can enhance signal-to-noise in complex lysates from erythroid and megakaryocytic lineages.
Translational Relevance and Future Research Directions
- Monitor dynamic beta-3 integrin surface expression during disease progression and therapeutic intervention with targeted agents.
- Integrate cd61 integrin beta 3 antibody functional grade 16061182 into organoid models to assess endothelial barrier function under shear stress.
- Leverage the antibody in high-content imaging platforms for quantitative phenotypic screens in adhesion and invasion assays.
- Explore paired multiplex strategies with alpha subunit markers to resolve tissue-specific integrin repertoires in heterogeneous specimens.
FAQ
Reader questions
What sample types are compatible with cd61 integrin beta 3 antibody functional grade 16061182 in flow cytometry?
Human and mouse peripheral blood mononuclear cells, endothelial cells, megakaryocyte cultures, and fixed or frozen tissue sections have been validated for reliable staining with this reagent.
Does this antibody recognize both alpha-IIb beta-3 and alpha-V beta-3 complexes?
Yes, the 16061182 clone detects conformational determinants present in both integrin subtypes, supporting studies of platelet aggregation and endothelial cell migration.
How should I adjust incubation times for intracellular staining with this antibody?
Permeabilization with saponin for 10–20 minutes followed by 20–30 minute primary incubation at room temperature or 4°C typically yields optimal specific signal without increased background.
Can this functional grade antibody be used in multiplex imaging panels with secondary detection?
Absolutely; the purified IgG format is compatible with species- and isotype-specific conjugated secondary reagents, and spectral unmixing strategies further reduce channel spillover in high-plex experiments.